Vacenovice is situated on flat to slightly hilly area, bounded on the south-east by the alluvial plain of the Morava River. The geological substrate of this area consists of aeolian sands. There are mostly pine forests, only on a few places there are preserved fragments of deciduous trees.
Typical species of flora include grasses - Calamagrostis epigejos (common name wood small-reed), Festuca psammophila, Corynephorus canescens (common name Grey Hair-grass), you can find here for example this herbs: Armeria vulgaris, Jasione montana, Thymus serpyllum (common name Breckland thyme). You can find here this rare species too: Stipa borysthenica (the only location in the Czech Republic), Verbascum phoeniceum, Carex stenophylla.
The uniqueness of the territory is also evident in the composition of fauna. You can find here mainly butterflies: Aplocera efformata (known as the Lesser Treble-bar), Zygaena laeta (known as the bloodword burnet), Schinia cognata, Chamaesphecia leucopsiformis, Sphinx pinastri (known as the pine hawk-moth), Hipparchia statilinus (known as the Tree Grayling). Reptiles are here represented by a total of five species - Lacerta agilis (known as the sand lizard) and Lacerta viridis (known as the European green lizard), Anguis fragilis (known as the slow worm), Natrix natrix (known as the Grass snake).
There was found abundant presence of amphibians in the newly restored small pond in Vacenovice - mainly Rana arvalis (known as the moor frog). Birds are found here in about 90 species, of which 75 are nesting here too: Upupa epops (known as the hoopoe), Lullula arborea (known as the woodlark), Ciconia nigra (known as the black stork), Falco subbuteo (known as the Eurasian hobby) and Caprimulgus europaeus (known as the European nightjar), Periparus ater (known as the coal tit) and Lophophanes cristatus (known as the European crested tit). The mammals are on aeolian sands represented by quarry. Interesting is the presence of bats too.